A MAP of tumor-host interactions in glioma at single cell resolution

Caruso, F.; Garofano, L.; D'Angelo, F.; Yu, K.; Tang, F.; Yuan, J.; Zhang, J.; Cerulo, L.; Bedognetti, D.; Sims, P.; Suva, M.; Su, X.-D.; Lasorella, A.; Iavarone, A.; Ceccarelli, M.

doi:10.1101/827758

bioRxiv · 10.1101/827758

A MAP of tumor-host interactions in glioma at single cell resolution

Caruso, F.·Garofano, L.·D'Angelo, F.·Yu, K.·Tang, F.·Yuan, J.·Zhang, J.·Cerulo, L.·Bedognetti, D.·Sims, P.·Suva, M.·Su, X.-D.·Lasorella, A.·Iavarone, A.·Ceccarelli, M.

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Abstract

Single-cell RNA sequencing is the reference technique to characterize the heterogeneity of tumor microenvironment and can be efficiently used to discover cross-talk mechanisms between immune cells and cancer cells. We present a novel method, single cell Tumor-Host Interaction tool (scTHI), to identify significantly activated ligand-receptor interactions across clusters of cells from single-cell RNA sequencing data. We apply our approach to uncover the ligand-receptor interactions in glioma using six publicly available human glioma datasets encompassing 71 patients. We provide a comprehensive map of the signalling mechanisms between malignant cells and non-malignant cells in glioma uncovering potential novel therapeutic targets.

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Caruso, F., Garofano, L., D'Angelo, F., Yu, K., Tang, F., Yuan, J., Zhang, J., Cerulo, L., Bedognetti, D., Sims, P., Suva, M., Su, X.-D., Lasorella, A., Iavarone, A., Ceccarelli, M.. 2019-11-01. A MAP of tumor-host interactions in glioma at single cell resolution. https://doi.org/10.1101/827758

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