Caramel Dye IV Induces Oxidative Stress Damage In Liver And Kidney From Mice

Abstract

Caramel dye IV (C-IV) is a synthetic organic product, does not present nutritional, ergogenic, or technological factors, but leads to reactive oxygen species (ROS), causing damage to a wide range of molecules, leading to cancer, cardiovascular and neurodegenerative diseases development. We aimed to verify the effects of different doses of C-IV dye on the markers of oxidative stress in the liver and kidneys from male Swiss CF-1 mice, divided into 4 experimental groups: control; C-IV 0.3g/kg; C-IV 1g/kg and C-IV 3g/kg. We found that 3 g/Kg of C-IV dye promote oxidative damage in liver and kidney homogenates, evidenced by the increase of lipid peroxidation, reduction of free SH groups, and higher ROS production. As a consequence, increased superoxide dismutase and acetylcholinesterase enzymes activities were detected. These damages were confirmed through histology images. These results indicate that daily doses might induce oxidative stress damages and possible lead to chronic diseases development.