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bioRxiv · 10.1101/821835

Torsin ATPases are required to complete nuclear pore complex biogenesis in interphase

Rampello, A. J.·Laudermilch, E.·Vishnoi, N.·Prohet, S. M.·Shao, L.·Zhao, C.·Lusk, C. P.·Schlieker, C.
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Abstract

Nuclear envelope herniations (blebs) containing FG-nucleoporins and ubiquitin are the phenotypic hallmark of Torsin ATPase manipulation. Both the dynamics of blebbing and the connection to nuclear pore biogenesis remain poorly understood. We employ a proteomics-based approach to identify MLF2 as a luminal component of the bleb. Using an MLF2-based live cell imaging platform, we demonstrate that NE blebbing occurs rapidly and synchronously immediately after nuclear envelope reformation during mitosis. Bleb formation is independent of ubiquitin conjugation within the bleb, but strictly dependent on POM121, a transmembrane nucleoporin essential for interphase nuclear pore biogenesis. Nup358, a late marker for interphase nuclear pore complex (NPC) biogenesis, is underrepresented relative to FG nucleoporins in nuclear envelopes of Torsin-deficient cells. The kinetics of bleb formation, its dependence on POM121, and a reduction of mature NPCs in Torsin deficient cells lead us to conclude that the hallmark phenotype of Torsin manipulation represents the accumulation of stalled NPC assembly intermediates.

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Rampello, A. J., Laudermilch, E., Vishnoi, N., Prohet, S. M., Shao, L., Zhao, C., Lusk, C. P., Schlieker, C.. 2019-10-29. Torsin ATPases are required to complete nuclear pore complex biogenesis in interphase. https://doi.org/10.1101/821835

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