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bioRxiv · 10.1101/811125

Inhibitory signaling in mammalian olfactory transduction potentially mediated by Gαo

Corey, E. A.·Ukhanov, K.·Bobkov, Y. V.·McIntyre, J. C.·Martens, J. R.·Ache, B. W.
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Abstract

Olfactory GPCRs (ORs) in mammalian olfactory receptor neurons (ORNs) mediate excitation through the Gs family member Golf. Here we tentatively associate a second G protein, Go, with inhibitory signalling in mammalian olfactory transduction by first showing that odor evoked phosphoinositide 3-kinase (PI3K)-dependent inhibition of signal transduction is absent in the native ORNs of mice carrying a conditional OMP-Cre based knockout of Go. We then identify an OR from native rat ORNs that are activated by octanol through cyclic nucleotide signaling and inhibited by citral in a PI3K-dependent manner. We show that the OR activates cyclic nucleotide signaling and PI3K signaling in a manner that reflects its functionality in native ORNs. Our findings lay the groundwork to explore the interesting possibility that ORs can interact with two different G proteins in a functionally identified, ligand-dependent manner to mediate opponent signaling in mature mammalian ORNs.

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Corey, E. A., Ukhanov, K., Bobkov, Y. V., McIntyre, J. C., Martens, J. R., Ache, B. W.. 2019-10-21. Inhibitory signaling in mammalian olfactory transduction potentially mediated by Gαo. https://doi.org/10.1101/811125

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