Exaiptasia diaphana from the Great Barrier Reef: a valuable resource for coral symbiosis research

Dungan, A. M.; Hartman, L. M.; Tortorelli, G.; Belderok, R.; Lamb, A. M.; Pisan, L.; McFadden, G. I.; Blackall, L. L.; van Oppen, M. J. H.

doi:10.1101/775510

bioRxiv · 10.1101/775510

Exaiptasia diaphana from the Great Barrier Reef: a valuable resource for coral symbiosis research

Dungan, A. M.·Hartman, L. M.·Tortorelli, G.·Belderok, R.·Lamb, A. M.·Pisan, L.·McFadden, G. I.·Blackall, L. L.·van Oppen, M. J. H.

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Abstract

The sea anemone, Exaiptasia diaphana, commonly known as Exaiptasia pallida or Aiptasia pallida, has become increasingly popular as a model for cnidarian-microbiome symbiosis studies due to its relatively rapid growth, ability to reproduce sexually and asexually, and symbiosis with diverse prokaryotes and the same microalgal symbionts (family Symbiodiniaceae) as its coral relatives. Clonal E. diaphana strains from Hawaii, the Atlantic Ocean, and Red Sea are now established for use in research. Here, we introduce Great Barrier Reef (GBR)-sourced E. diaphana strains as additions to the model repertoire. Sequencing of the 18S rRNA gene confirmed the anemones to be E. diaphana while genome-wide single nucleotide polymorphism analysis revealed four distinct genotypes. Based on Exaiptasia-specific inter-simple sequence repeat (ISSR)-derived sequence characterized amplified region (SCAR) marker and gene loci data, these four E. diaphana genotypes are distributed across several divergent phylogenetic clades with no clear phylogeographical pattern. The GBR E. diaphana genotypes comprised three females and one male, which all host Breviolum minutum as their homologous Symbiodiniaceae endosymbiont. When acclimating to an increase in light levels from 12 to 28 mol photons m-2 s-1, the genotypes exhibited significant variation in maximum quantum yield of Symbiodiniaceae photosystem II and Symbiodiniaceae cell density. The comparatively high levels of physiological and genetic variability among GBR anemone genotypes makes these animals representative of global E. diaphana diversity and thus excellent model organisms. The addition of these GBR strains to the worldwide E. diaphana collection will contribute to cnidarian symbiosis research, particularly in relation to the climate resilience of coral reefs.

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Dungan, A. M., Hartman, L. M., Tortorelli, G., Belderok, R., Lamb, A. M., Pisan, L., McFadden, G. I., Blackall, L. L., van Oppen, M. J. H.. 2019-09-20. Exaiptasia diaphana from the Great Barrier Reef: a valuable resource for coral symbiosis research. https://doi.org/10.1101/775510

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