Progressive differentiation of memory CD8 T cells in the cervicovaginal tissue

Dave, V.; Cardozo Ojeda, E. F.; Mair, F.; Erickson, J.; Woodward-Davis, A.; Soerens, A.; Czartoski, J.; Teague, C.; Oberle, S.; Zehn, D.; Schiffer, J.; Lund, J. M.; Prlic, M.

doi:10.1101/769711

bioRxiv · 10.1101/769711

Progressive differentiation of memory CD8 T cells in the cervicovaginal tissue

Dave, V.·Cardozo Ojeda, E. F.·Mair, F.·Erickson, J.·Woodward-Davis, A.·Soerens, A.·Czartoski, J.·Teague, C.·Oberle, S.·Zehn, D.·Schiffer, J.·Lund, J. M.·Prlic, M.

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Abstract

Tissue-resident memory CD8 T cells (CD8 TRM) are critical for maintaining barrier immunity. CD8 TRM have been mainly studied in the skin and gut with recent studies suggesting that the signals that control tissue-residence and phenotype are highly tissue-dependent. We examined the T cell compartment in healthy human cervicovaginal tissue (CVT) and found that most CD8 T cells were granzyme B+ and TCF-1-. To address if this phenotype is driven by CVT tissue-residence, we used a mouse model to control for environmental factors. Using localized and systemic infection models, we found that CD8 TRM in the mouse CVT gradually acquired a granzyme B+, TCF-1- phenotype as seen in human CVT. In contrast to CD8 TRM in the gut, these CD8 TRM were not stably maintained regardless of the initial infection route, which led to reductions in local immunity. Our data show that residence in the CVT is sufficient to progressively shape the size and function of its CD8 TRM compartment. SummaryThe tissue-resident memory (TRM) CD8 T cell compartment in human and mouse cervicovaginal tissue (CVT) is remarkably similar. The CVT TRM compartment is maintained autonomously and does not reach phenoypical or numerical equilibrium. The numerical decline leads to impaired viral control in a secondary challenge.

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Dave, V., Cardozo Ojeda, E. F., Mair, F., Erickson, J., Woodward-Davis, A., Soerens, A., Czartoski, J., Teague, C., Oberle, S., Zehn, D., Schiffer, J., Lund, J. M., Prlic, M.. 2019-09-14. Progressive differentiation of memory CD8 T cells in the cervicovaginal tissue. https://doi.org/10.1101/769711

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