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bioRxiv · 10.1101/768119

DNA repair by Rad52 liquid droplets

Oshidari, R.·Huang, R.·Medghalchi, M.·Tse, E. Y. W.·Ashgriz, N.·Lee, H. O.·Wyatt, H.·Mekhail, K.
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Abstract

Cellular processes are influenced by liquid phase separation, but its role in DNA repair is unclear. Here, we show that in Saccharomyces cerevisiae, Rad52 DNA repair proteins at different DNA damage sites assemble liquid droplets that fuse into a repair centre droplet. This larger droplet concentrates tubulin and projects short aster-like microtubule filaments, which tether the droplet to longer microtubule filaments mediating the mobilization of damaged DNA to the nuclear periphery for repair.

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Oshidari, R., Huang, R., Medghalchi, M., Tse, E. Y. W., Ashgriz, N., Lee, H. O., Wyatt, H., Mekhail, K.. 2019-09-13. DNA repair by Rad52 liquid droplets. https://doi.org/10.1101/768119

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