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bioRxiv · 10.1101/765958

One-Shot phase-recovery using a Cellphone RGB Camera on a Jamin-Lebedeff Microscope

Diederich, B.·Marsikova, B.·Amos, B.·Heintzmann, R.
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Abstract

Jamin-Lebedeff (JL) polarization interference microscopy is a classical method for determining the change in the optical path of transparent tissues. Whilst a differential interference contrast (DIC) microscopy interferes an image with itself shifted by half a point spread function, the shear between the object and reference image in a JL-microscope is about half the field of view. The optical path difference (OPD) between the sample and reference region (assumed to be empty) is encoded into a colour by white-light interference. From a colour-table, the Michel-Levy chart, the OPD can be deduced. In cytology JL-imaging can be used as a way to determine the OPD which closely corresponds to the dry mass per area of cells in a single image.\n\nLike in other interference microscopy methods (e.g. holography), we present a phase retrieval method relying on single-shot measurements only, thus allowing real-time quantitative phase measurements. This is achieved by adding several customized 3D-printed parts (e.g. rotational polarization-filter holders) and a modern cellphone with an RGB-camera to the Jamin-Lebedeff setup, thus bringing an old microscope back to life.\n\nThe algorithm is calibrated using a reference image of a known phase object (e.g. optical fiber). A gradient-descent based inverse problem generates an inverse look-up-table (LUT) which is used to convert the measured RGB signal of a phase-sample into an OPD.\n\nTo account for possible ambiguities in the phase-map or phase-unwrapping artifacts we introduce a total-variation based regularization. We present results from fixed and living biological samples as well as reference samples for comparison.

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Diederich, B., Marsikova, B., Amos, B., Heintzmann, R.. 2019-09-12. One-Shot phase-recovery using a Cellphone RGB Camera on a Jamin-Lebedeff Microscope. https://doi.org/10.1101/765958

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