Draft genome sequence of a popular Indian tea genotype TV-1

Mondal, T. K.; Rawal, H. C.; Bera, B.; Mohan Kumar, P.; Choubey, M.; Saha, G.; Das, B.; Bandyopadhyay, T.; Ilango, R. V. J.; Sharma, T. R.; Barua, A.; Radhakrishnan, B.; Singh, N. K.

doi:10.1101/762161

bioRxiv · 10.1101/762161

Draft genome sequence of a popular Indian tea genotype TV-1

Mondal, T. K.·Rawal, H. C.·Bera, B.·Mohan Kumar, P.·Choubey, M.·Saha, G.·Das, B.·Bandyopadhyay, T.·Ilango, R. V. J.·Sharma, T. R.·Barua, A.·Radhakrishnan, B.·Singh, N. K.

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Abstract

Camellia assamica L. (O). Kuntze. var. TV-1 from Assam, India is one of the most important tea cultivar of Indian Tea Industry which botanically belongs to Assam type and has distinct morphological as well as chemical constituent in leaf than China type of tea. We here present the first draft genome sequence of TV-1, assembled in 2.93 Gb size comprising of 14,824 scaffolds and covering 97.66% of the predicted genome size (3.0 Gb) as determined by flow cytometry. The N50 value, longest and average contig size for this draft genome was found to be as 538.96 Kb, 3.64 Mb and 197.99 Kb, respectively. There were 495,747 SSRs (Di- to Hexa-mers) in the genome and the repeat contents accounted for 71.87% of the genome. About 94.40% genome completeness was predicted with BUSCO, maximum among the published and reported tea genomes. The clustering of the assembled genome with Hi-C data resulted in 15 clusters ranging from the size of 631.46 Mb to 14.39 Mb and accounting 99.32% of assembled genome. This data will act as the starting point for unravelling the important genes and differentiating Indian tea from other commercially available tea varieties.

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Mondal, T. K., Rawal, H. C., Bera, B., Mohan Kumar, P., Choubey, M., Saha, G., Das, B., Bandyopadhyay, T., Ilango, R. V. J., Sharma, T. R., Barua, A., Radhakrishnan, B., Singh, N. K.. 2019-09-09. Draft genome sequence of a popular Indian tea genotype TV-1. https://doi.org/10.1101/762161

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