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bioRxiv · 10.1101/746479

Single-cell analysis of intestinal immune cells during helminth infection

Ferrer-Font, L.·Mehta, P.·Harmos, P.·Schmidt, A.·Price, K. M.·Hermans, I. F.·Ronchese, F.·Le Gros, G.·Mayer, J. U.
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Abstract

Single cell isolation from helminth infected intestines has been notoriously difficult, due to the strong anti-parasite type 2 immune responses that drive mucus production, tissue remodeling and immune cell infiltration. Through the systematic optimization of a standard intestinal digestion protocol, we were able to isolate millions of immune cells from heavily infected tissues. Using this protocol, we validated many hallmarks of anti-parasite immunity and analyzed immune cells from the lamina propria and granulomas during helminth development, as well as acute and chronic worm infection.

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Ferrer-Font, L., Mehta, P., Harmos, P., Schmidt, A., Price, K. M., Hermans, I. F., Ronchese, F., Le Gros, G., Mayer, J. U.. 2019-08-24. Single-cell analysis of intestinal immune cells during helminth infection. https://doi.org/10.1101/746479

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