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bioRxiv · 10.1101/738013

Release of stem cells from quiescence reveals multiple gliogenic domains in the adult brain

Delgado, A.·Maldonado-Soto, A.·Silva-Vargas, V.·Mizrak, D.·von Kaenel, T.·Paul, A.·Madar, A.·Cuervo, H.·Kitajewski, J.·Lin, C.-S.·Doetsch, F.
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Abstract

Quiescent neural stem cells (NSCs) in the adult ventricular-subventricular zone (V-SVZ) have a regional identity and undergo activation to generate neurons. The domains for gliogenesis are less explored. Here we show that Platelet-Derived Growth Factor Receptor beta (PDGFR{beta}) is expressed by adult V-SVZ NSCs that generate olfactory bulb interneurons and glia with slow baseline kinetics. Selective deletion of PDGFR{beta} in adult V-SVZ NSCs leads to their release from quiescence uncovering multiple domains in the septal wall for oligodendrocyte and astrocyte formation. Unexpectedly, we identify a novel intraventricular oligodendrocyte progenitor inside the brain ventricles. Together our findings reveal different NSC spatial domains for gliogenesis in the adult V-SVZ that are largely quiescent under homeostasis and may have key functions for brain plasticity.

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Delgado, A., Maldonado-Soto, A., Silva-Vargas, V., Mizrak, D., von Kaenel, T., Paul, A., Madar, A., Cuervo, H., Kitajewski, J., Lin, C.-S., Doetsch, F.. 2019-08-16. Release of stem cells from quiescence reveals multiple gliogenic domains in the adult brain. https://doi.org/10.1101/738013

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