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bioRxiv · 10.1101/734475

Fascin regulates protrusions and delamination to promote invasive, collective cell migration in vivo.

Lamb, M. C.·Anliker, K. K.·Tootle, T. L.
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Abstract

Fascin is an actin bundling protein that is essential for developmental cell migrations and promotes cancer metastasis. In addition to bundling actin, Fascin has several actin-independent roles. Border cell migration during Drosophila oogenesis provides an excellent model to study Fascins various roles during invasive, collective cell migration. Border cell migration requires Fascin. Fascin functions not only within the migrating border cells, but also within the nurse cells, the substrate for this migration. Loss of Fascin results in increased, shorter and mislocalized protrusions during migration. Data supports the model that Fascin promotes the activity of Enabled, an actin elongating factor, to regulate migration. Additionally, loss of Fascin inhibits border cell delamination. These defects are partially due to altered E-cadherin localization in the border cells; this is predicted to be an actin-independent role of Fascin. Overall, Fascin is essential for multiple aspects of this invasive, collective cell migration, and functions in both actin-dependent and -independent manners. These findings have implications beyond Drosophila, as border cell migration has emerged as a model to study mechanisms mediating cancer metastasis.

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Lamb, M. C., Anliker, K. K., Tootle, T. L.. 2019-08-13. Fascin regulates protrusions and delamination to promote invasive, collective cell migration in vivo.. https://doi.org/10.1101/734475

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