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bioRxiv · 10.1101/732610

Cellular metabolic activity marker via selective turn-ON detection of serum albumin using an NBD-based fluoroprobe

Dutta, T.·Pal, K.·Koner, A.
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Abstract

A nitrobenzoxadiazole-based fluoroprobe (NBD-Bu) is designed to probe cellular metabolic activity in cancer and normal cells. NBD-Bu shows a significant fluorescence enhancement upon selective binding to serum albumin. The site specificity of NBD-Bu has been explored through a competitive displacement assay in the presence of site-specific markers such as warfarin and ibuprofen. Subsequently, high-resolution fluorescence microscopy results consolidated the potential of NBD-Bu for cellular imaging and detection of abnormal cellular metabolic activity.

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Dutta, T., Pal, K., Koner, A.. 2019-08-12. Cellular metabolic activity marker via selective turn-ON detection of serum albumin using an NBD-based fluoroprobe. https://doi.org/10.1101/732610

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