MKPV (aka MuCPV) and related chapparvoviruses are nephro-tropic and encode novel accessory proteins p15 and NS2

Jolly, C. J.; Lee, Q.; Padula, M. P.; Pinello, N.; Williams, S. H.; O'Rourke, M. B.; Fumagalli, M. J.; Orkin, J. D.; Shaban, B.; Brenner, O.; Weninger, W.; Souza, W. M. d.; Melin, A. D.; Wong, J. J.- L.; Crim, M. J.; Monette, S.; Roediger, B.

doi:10.1101/732537

bioRxiv · 10.1101/732537

MKPV (aka MuCPV) and related chapparvoviruses are nephro-tropic and encode novel accessory proteins p15 and NS2

Jolly, C. J.·Lee, Q.·Padula, M. P.·Pinello, N.·Williams, S. H.·O'Rourke, M. B.·Fumagalli, M. J.·Orkin, J. D.·Shaban, B.·Brenner, O.·Weninger, W.·Souza, W. M. d.·Melin, A. D.·Wong, J. J.- L.·Crim, M. J.·Monette, S.·Roediger, B.

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Abstract

Mouse kidney parvovirus (MKPV) is a member of the provisional Chapparvovirus genus that causes renal disease in immune-compromised mice, with a disease course reminiscent of polyomavirus-associated nephropathy in immune-suppressed kidney transplant patients. Here we map four MKPV transcripts, created by alternative splicing, to a common transcription initiation region, and use mass spectrometry to identify \"p10\" and \"p15\" as novel chapparvovirus accessory proteins produced in MKPV-infected kidneys. p15 and a splicing-dependent putative accessory protein NS2 are conserved in all near-complete tetrapod chapparvovirus genomes currently available (from mammals, birds and a reptile). In contrast, p10 may be encoded only by viruses with >60% amino acid identity to MKPV. We show that MKPV is kidney-tropic and that the bat chapparvovirus DrPV-1 and a non-human primate chapparvovirus, CKPV, are also found in the kidneys of their hosts. We propose, therefore, that chapparvoviruses with >60% VP1 amino acid identity to MKPV be classified into a genus dubbed Nephroparvovirus, which is consistent with nomenclature for the genus Erythroparvovirus.

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Jolly, C. J., Lee, Q., Padula, M. P., Pinello, N., Williams, S. H., O'Rourke, M. B., Fumagalli, M. J., Orkin, J. D., Shaban, B., Brenner, O., Weninger, W., Souza, W. M. d., Melin, A. D., Wong, J. J.- L., Crim, M. J., Monette, S., Roediger, B.. 2019-08-15. MKPV (aka MuCPV) and related chapparvoviruses are nephro-tropic and encode novel accessory proteins p15 and NS2. https://doi.org/10.1101/732537

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