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bioRxiv · 10.1101/729251

Structural assembly of the human Miro1/2 GTPases based on the crystal structure of the N-terminal GTPase domain

Smith, K. P.·Focia, P. J.·Chakravarthy, S.·Landahl, E. C.·Klosowiak, J. L.·Rice, S. E.·Freymann, D. M.
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Abstract

Dysfunction in mitochondrial dynamics is believed to contribute to a host of neurological disorders and has recently been implicated in cancer metastasis. The outer mitochondrial membrane adapter protein Miro functions in the regulation of mitochondrial mobility and degradation, however, the structural basis for its roles in mitochondrial regulation remain unknown. Here, we report a 1.7[A] crystal structure of N-terminal GTPase domain (nGTPase) of human Miro1 bound unexpectedly to GTP, thereby revealing a non-catalytic configuration of the putative GTPase active site. We identify two conserved surfaces of the nGTPase, the \"SELFYY\" and \"ITIP\" motifs, that are potentially positioned to mediate dimerization or interaction with binding partners. Additionally, we report small angle X-ray scattering (SAXS) data obtained from the intact soluble HsMiro1 and its paralog HsMiro2. Taken together, the data allow modeling of a crescent-shaped assembly of the full-length soluble domain of HsMiro1/2.\n\nPDB referenceCrystal structure of the human Miro1 N-terminal GTPase bound to GTP, 6D71

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Smith, K. P., Focia, P. J., Chakravarthy, S., Landahl, E. C., Klosowiak, J. L., Rice, S. E., Freymann, D. M.. 2019-08-08. Structural assembly of the human Miro1/2 GTPases based on the crystal structure of the N-terminal GTPase domain. https://doi.org/10.1101/729251

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