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bioRxiv · 10.1101/714907

cis dominantly explains regulatory divergence between two indica rice genotypes; drought further enhances regulatory differences

Ereful, N. C.·Liu, L.-y.·Kao, S.-M.·Tsai, E.·Laurena, A.·Thomson, M.·Greenland, A.·Powell, W.·Mackay, I.·Leung, H.
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Abstract

Abstract cis and/or trans regulatory divergence within or between related taxa on a genome-wide scale has been largely unexamined in crops, more so, the effect of stress on cis/trans architecture. In this study, the indica genotypes IR64, an elite drought-susceptible lowland variety, and Apo (IR55423-01 or NSIC RC9), a moderate drought-tolerant upland genotype together with their hybrid (IR64 x Apo) were exposed to non- and water-stress conditions. Evidence of cis and/or trans regulatory differences was tested between these two indica rice genotypes. By sequencing (RNA-seq) the parents and their hybrid, we are able to map genes diverging in cis and/or trans factors between the two genotypes. Under non-stress conditions, cis dominantly explains (11.2%) regulatory differences, followed by trans (8.9%). Further analysis showed that water-limiting conditions largely affect trans and cis + trans factors. Between the two inbred lines, Apo appears to exhibit higher expression fold change of genes enriched in "response to stress" and "photosynthesis" under non- and water-stress conditions. On the molecular level, cis and/or trans regulatory divergence explains their genotypic differences and differential drought response. Parent-hybrid RNA-seq has the potential to identify genes diverging in cis and/or trans factors even between intra-sub-specifically related genotypes. By comparing cis/trans landscape under stressed and unstressed conditions, this approach has the ability to assess the impact of drought on gene expression. Computational analysis and association of several drought-yield QTL markers with cis-diverging genes provide converging evidences suggestive of a potential approach to identify trait-associated candidate genes using hybrids and their parents alone. Key Messagecis dominantly explains divergence of two indica rice genotypes, IR64 and Apo under normal conditions while trans and cis + trans regulatory factors are largely affected by drought

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Ereful, N. C., Liu, L.-y., Kao, S.-M., Tsai, E., Laurena, A., Thomson, M., Greenland, A., Powell, W., Mackay, I., Leung, H.. 2019-07-25. cis dominantly explains regulatory divergence between two indica rice genotypes; drought further enhances regulatory differences. https://doi.org/10.1101/714907

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