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bioRxiv · 10.1101/713545

Targeting DNA damage response and Replication Stress in Pancreatic Cancer

Abstract

Continuing recalcitrance to therapy cements pancreatic cancer (PC) as the most lethal malignancy, which is set to become the second leading cause of cancer death in our society. We interrogated the transcriptome, genome, proteome and functional characteristics of 61 novel PC patient-derived cell lines to define novel therapeutic strategies targeting the DNA damage response (DDR) and replication stress. We show that patient-derived cell lines faithfully recapitulate the epithelial component of pancreatic tumors including previously described molecular subtypes. Biomarkers of DDR deficiency, including a novel signature of homologous recombination deficiency, co-segregates with response to platinum and PARP inhibitor therapy in vitro and in vivo. We generated a novel signature of replication stress with potential clinical utility in predicting response to ATR and WEE1 inhibitor treatment. Replication stress and DDR deficiency are independent of each other, creating opportunities for therapy in DDR proficient PC, and post-platinum therapy.\n\n\n\nO_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=145 SRC=\"FIGDIR/small/713545v1_ufig1.gif\" ALT=\"Figure 1\">\nView larger version (47K):\norg.highwire.dtl.DTLVardef@106f2b8org.highwire.dtl.DTLVardef@1294d22org.highwire.dtl.DTLVardef@86f5d3org.highwire.dtl.DTLVardef@751e13_HPS_FORMAT_FIGEXP M_FIG C_FIG STATEMENT OF SIGNIFICANCEWe define therapeutic strategies that target subgroups of PC using novel signatures of DNA damage response deficiency and replication stress. This potentially offers patients with DNA repair defects therapeutic options outside standard of care platinum chemotherapy and is being tested in clinical trials on the Precision-Panc platform.

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BibTeXRIS

Dreyer, S., Upstill-Goddard, R., Paulus-Hock, V., Paris, C., Maria-Lampraki, E., Dray, E., Serrels, B., Rebus, S., Brunton, H., Nourse, C., Bailey, U.-M., Jones, M., Moran-Jones, K., Wright, D., Duthie, F., Oien, K., Evers, L., McKay, C., McGregor, G., Gulati, A., Brough, R., Bajrami, I., Pettitt, S., Dzubiunski, M., Candido, J., Balkwill, F., Barry, S., Grutzmann, R., Rahib, L., Glasgow Precision Oncology Laboratory,, Australian Pancreatic Genome Initiative,, Johns, A., Pajic, M., Froeling, F., Beer, P., Musgrove, E., Petersen, G., Ashworth, A., Frame, M., Crawford, H., Simeone, D., Lord,. 2019-07-24. Targeting DNA damage response and Replication Stress in Pancreatic Cancer. https://doi.org/10.1101/713545

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