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bioRxiv · 10.1101/712687

Urbilaterian origin and evolution of sNPF-type neuropeptide signalling

Yanez Guerra, L. A.·Zhong, X.·Moghul, I.·Butts, T.·Zampronio, C.·Jones, A. M.·Mirabeau, O.·Elphick, M. R.
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Abstract

Physiology and behaviour are controlled by neuropeptide signalling systems comprising peptide ligands and cognate receptors. Molecular phylogenetics combined with experimental identification of neuropeptide-receptor pairs has revealed that many neuropeptide signalling systems originated in the urbilaterian common ancestor of protostomes and deuterostomes. Neuropeptide-Y/neuropeptide-F (NPY/NPF)-type signalling is one such example, whereas NPY/NPF-related short-NPF (sNPF)-type signalling has hitherto only been identified in protostomes. Here we report the discovery of a neuropeptide (pQDRSKAMQAERTGQLRRLNPRF-NH2) that is the ligand for an sNPF-type receptor in a deuterostome, the starfish Asterias rubens (Phylum Echinodermata). Informed by phylogenetic analysis of sequence data, we conclude that the paralogous NPY/NPF-type and sNPF-type signalling systems originated in Urbilateria but NPY/NPF-type signalling was lost in echinoderms. Furthermore, we present evidence that sNPF-type peptides are orthologs of vertebrate prolactin-releasing peptides. Our findings demonstrate the importance of experimental studies on echinoderms for reconstructing the evolutionary history of neuropeptide signalling systems.

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Yanez Guerra, L. A., Zhong, X., Moghul, I., Butts, T., Zampronio, C., Jones, A. M., Mirabeau, O., Elphick, M. R.. 2019-07-24. Urbilaterian origin and evolution of sNPF-type neuropeptide signalling. https://doi.org/10.1101/712687

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