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bioRxiv · 10.1101/711515

The proteasome regulator PI31 is required for protein homeostasis, synapse maintenance and neuronal survival in mice

Minis, A.·Rodriguez, J. A.·Levin, A.·Liu, K.·Govek, E.-E.·Hatten, M. E.·Steller, H.
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Abstract

Proteasome-mediated degradation of intracellular proteins is essential for cell function and survival. The proteasome-binding protein PI31 (Proteasomal Inhibitor of 31kD) promotes 26S assembly and functions as an adapter for proteasome transport in axons. As localized protein synthesis and degradation is especially critical in neurons, we generated a conditional loss of PI31 in spinal motor neurons (MNs) and cerebellar Purkinje cells (PCs). A cKO of PI31 in these neurons caused axon degeneration, neuronal loss and progressive spinal and cerebellar neurological dysfunction. For both MNs and PCs, markers of proteotoxic stress preceded axonal degeneration and motor dysfunction, indicating a critical role for PI31 in neuronal homeostasis. The time course of the loss of MN and PC function in developing mouse CNS suggests a key role for PI31 in human developmental neurological disorders.\n\nStatement of SignificanceThe conserved proteasome-binding protein PI31 serves as an adapter to couple proteasomes with cellular motors to mediate their transport to distal tips of neurons where protein breakdown occurs. We generated global and conditional PI31 knockout mouse strains and show that this protein is required for protein homeostasis, and that its conditional inactivation in neurons disrupts synaptic structures and long-term survival. This work establishes a critical role for PI31 and local protein degradation in the maintenance of neuronal architecture, circuitry and function. Because mutations that impair PI31 function cause neurodegenerative diseases in humans, reduced PI31 activity may contribute to age-related neurodegenerative diseases.

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Minis, A., Rodriguez, J. A., Levin, A., Liu, K., Govek, E.-E., Hatten, M. E., Steller, H.. 2019-07-22. The proteasome regulator PI31 is required for protein homeostasis, synapse maintenance and neuronal survival in mice. https://doi.org/10.1101/711515

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