Probing protein-protein and protein-substrate interactions in the dynamic membrane-associated ternary complex of Cytochromes P450, b5, and Reductase

Gentry, K.; Anantharamaiah, G. M.; Ramamoorthy, A.

doi:10.1101/707729

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bioRxiv · 10.1101/707729

Probing protein-protein and protein-substrate interactions in the dynamic membrane-associated ternary complex of Cytochromes P450, b5, and Reductase

Gentry, K.·Anantharamaiah, G. M.·Ramamoorthy, A.

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Abstract

Cytochrome P450 (cytP450) interacts with two redox partners, cytP450 reductase and cytochrome-b5, to metabolize substrates. Using NMR, we reveal changes in the dynamic interplay when all three proteins are incorporated into lipids nanodiscs in the absence and presence of substrates.

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Gentry, K., Anantharamaiah, G. M., Ramamoorthy, A.. 2019-07-19. Probing protein-protein and protein-substrate interactions in the dynamic membrane-associated ternary complex of Cytochromes P450, b5, and Reductase. https://doi.org/10.1101/707729

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