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bioRxiv · 10.1101/707463

Radial Glial from mammalian developing neocortex can divide symmetrically in vitro

Ledesma-Terron, M.·Peralta-Canadas, N.·Miguez, D. G.
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Abstract

Radial Glial progenitors in the mammalian developing neocortex have been shown to follow a deterministic differentiation program restricted to an asymmetric-only mode of division. This feature seems incompatible with their well known ability to expand in number when cultured in vitro, driven by Fibroblast Growth Factor 2 and other mitogenic signals. The changes in their differentiation dynamics that allow this transition from in vivo asymmetric-only division mode to an in vitro self-renewing culture have not been fully characterized. Here we combine experiments of Radial Glia cultures with theory and numerical models to show that Fibroblast Growth Factor 2 has a triple effect by simultaneously increasing the growth fraction, promoting symmetric divisions and shortening the length of the cell cycle. This combined effect of Fibroblast Growth Factor 2 in the differentiation dynamics of Radial Glial progenitors partner to establish and sustain a pool of rapidly proliferating in vitro pool of Radial Glial progenitors.

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Ledesma-Terron, M., Peralta-Canadas, N., Miguez, D. G.. 2019-07-18. Radial Glial from mammalian developing neocortex can divide symmetrically in vitro. https://doi.org/10.1101/707463

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