Combined inhibition of mTOR and PIKKs exploits replicative and checkpoint vulnerabilities to induce death of PI3K-activated triple-negative breast cancer cells

Chopra, S. S.; Jenney, A.; Palmer, A.; Niepel, M.; Chung, M.; Mills, C.; Sivakumaren, S. C.; Liu, Q.; Chen, J.-Y.; Yapp, C.; Asara, J.; Gray, N. S.; Sorger, P. K.

doi:10.1101/700625

bioRxiv · 10.1101/700625

Combined inhibition of mTOR and PIKKs exploits replicative and checkpoint vulnerabilities to induce death of PI3K-activated triple-negative breast cancer cells

Chopra, S. S.·Jenney, A.·Palmer, A.·Niepel, M.·Chung, M.·Mills, C.·Sivakumaren, S. C.·Liu, Q.·Chen, J.-Y.·Yapp, C.·Asara, J.·Gray, N. S.·Sorger, P. K.

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Abstract

SUMMARY SUMMARY HIGHLIGHTS INTRODUCTION RESULTS DISCUSSION FUNDING AUTHOR CONTRIBUTIONS STAR METHODS Cell lines METHOD DETAILS Western blotting Evaluation of drug sensitivity Immunofluorescence microscopy Primary antibodies and dilutions Single-stranded DNA ssDNA and... Live cell microscopy Extraction of polar metabolites Metabolomics profiling by... Isobolograms and analysis of... Cell cycle model and... QUANTIFICATION AND STATISTICAL... Viable cell counts ...

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Chopra, S. S., Jenney, A., Palmer, A., Niepel, M., Chung, M., Mills, C., Sivakumaren, S. C., Liu, Q., Chen, J.-Y., Yapp, C., Asara, J., Gray, N. S., Sorger, P. K.. 2019-07-12. Combined inhibition of mTOR and PIKKs exploits replicative and checkpoint vulnerabilities to induce death of PI3K-activated triple-negative breast cancer cells. https://doi.org/10.1101/700625

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