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bioRxiv · 10.1101/697433

Classification of hospital and urban wastewater resistome and microbiota over time and their relationship to the eco-exposome.

Buelow, E.·Rico, A.·Gaschet, M.·Lourenco, J.·Kennedy, S. P.·Wiest, L.·Ploy, M.-C.·Dagot, C.
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Abstract

Wastewaters (WW) are important sources for the dissemination of antimicrobial resistance (AMR) into the environment. Hospital WW (HWW) contain higher loads of micro-pollutants and AMR markers than urban WW (UWW). Little is known about the long-term dynamics of H and U WW and the impact of their joined treatment on the general burden of AMR. Here, we characterized the resistome, microbiota and eco-exposome signature of 126 H and U WW samples treated separately for three years, and then mixed, over one year. Multi-variate analysis and machine learning revealed a robust signature for each WW with no significant variation over time before mixing, and once mixed, both WW closely resembled U signatures. We demonstrated a significant impact of pharmaceuticals and surfactants on the resistome and microbiota of H and U WW. Our results present considerable targets for AMR related risk assessment of WW.

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Buelow, E., Rico, A., Gaschet, M., Lourenco, J., Kennedy, S. P., Wiest, L., Ploy, M.-C., Dagot, C.. 2019-07-09. Classification of hospital and urban wastewater resistome and microbiota over time and their relationship to the eco-exposome.. https://doi.org/10.1101/697433

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