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bioRxiv · 10.1101/687202

Dual-plane 3-photon microscopy with remote focusing

Takasaki, K. T.·Tsyboulski, D.·Waters, J.
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Abstract

3-photon excitation enables in vivo fluorescence microscopy deep in densely labeled and highly scattering samples. To date, 3-photon excitation has been restricted to scanning a single focus, limiting the speed of volume acquisition. Here, for the first time to our knowledge, we implemented and characterized dual-plane 3-photon microscopy with temporal multiplexing and remote focusing, and performed simultaneous in vivo calcium imaging of two planes deep in the cortex of a pan-excitatory GCaMP6s transgenic mouse. This method is a straightforward and generalizable modification to single-focus 3PE systems, doubling the rate of volume (column) imaging with off-the-shelf components and minimal technical constraints.

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Takasaki, K. T., Tsyboulski, D., Waters, J.. 2019-06-29. Dual-plane 3-photon microscopy with remote focusing. https://doi.org/10.1101/687202

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