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bioRxiv · 10.1101/683243

Retrograde mitochondrial transport is essential for mitochondrial homeostasis in neurons

Mandal, A.·Pinter, K.·Mosqueda, N.·Beirl, A.·Lomash, R. M.·Won, S.·Kindt, K. S.·Drerup, C. M.
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Abstract

Mitochondrial transport in neurons is essential for forming and maintaining axonal projections. While much is known about anterograde mitochondrial movement, the function of retrograde mitochondrial motility in neurons was unknown. We investigated the dynamics and utility of retrograde mitochondrial transport. Using long-term tracking of mitochondria in vivo, we found mitochondria in axon terminals turnover within hours via retrograde transport. Mitochondria do not return to the cell body solely for degradation; rather, mitochondria use bidirectional transport to redistribute themselves throughout the neuron. Disruption of retrograde mitochondrial transport severely depletes the cell body of mitochondria and impacts mitochondrial health throughout the cell. Altered mitochondrial health correlates with decreased synaptic activity. Using proteomics, we provide evidence that retrograde mitochondrial movement functions to maintain the organelles proteome. Together, our work demonstrates that mitochondrial retrograde transport is essential for the maintenance of a homeostatic population of mitochondria in neurons and consequently effective synaptic activity through promoting mitochondrial protein turnover.

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Mandal, A., Pinter, K., Mosqueda, N., Beirl, A., Lomash, R. M., Won, S., Kindt, K. S., Drerup, C. M.. 2019-06-27. Retrograde mitochondrial transport is essential for mitochondrial homeostasis in neurons. https://doi.org/10.1101/683243

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