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bioRxiv · 10.1101/676189

Tailoring cryo-electron microscopy grids by photo-micropatterning for in-cell structural studies

Toro-Nahuelpan, M.·Zagoriy, I.·Senger, F.·Blanchoin, L.·Thery, M.·Mahamid, J.
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Abstract

Spatially-controlled cell adhesion on electron microscopy (EM) supports remains a bottleneck in specimen preparation for cellular cryo-electron tomography. Here, we describe contactless and mask-free photo-micropatterning of EM grids for site-specific deposition of extracellular matrix-related proteins. We attained refined cell positioning for micromachining by cryo-focused ion beam milling. Complex patterns generated predictable intracellular organization, allowing direct correlation between cell architecture and in-cell 3D-structural characterization of the underlying molecular machinery.

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Toro-Nahuelpan, M., Zagoriy, I., Senger, F., Blanchoin, L., Thery, M., Mahamid, J.. 2019-06-19. Tailoring cryo-electron microscopy grids by photo-micropatterning for in-cell structural studies. https://doi.org/10.1101/676189

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