Caenorhabditis elegans Exhibits Positive Gravitaxis

Abstract

Whether or not the micro swimmer Caenorhabditis elegans senses and respond to gravity is unknown. We find that C. elegans aligns its swimming direction with that of the gravity vector (positive gravitaxis). When placed in an aqueous solution that is denser than the animals, they still orient downwards, indicating that non-uniform mass distribution and/or hydrodynamic effects are not responsible for animals downward orientation. Paralyzed worms and worms with globally disrupted sensory cilia do not change orientation as they settle in solution, indicating that gravitaxis is an active behavior that requires gravisensation. Other types of sensory driven orientation behaviors cannot explain our observed downward orientation. Like other neural behaviors, the ability to respond to gravity declines with age. Our study establishes gravitaxis in the micro swimmer C. elegans and suggests that C. elegans can be used as a genetically tractable system to study molecular and neural mechanisms of gravity sensing and orientation.\n\nSignificance StatementUnderstanding how animals respond to gravity is not only of fundamental scientific interest, but has clinical relevance, given the prevalence of postural instability in aged individuals. Determining whether C. elegans responds to gravity is important for mechanistic studies of gravity sensing in an experimentally tractable animal, for a better understanding of nematode ecology and evolution, and for studying biological effects of microgravity. Our experiments, which indicate that C. elegans senses and responds to gravity, set the stage for mechanistic studies on molecular mechanisms of gravity sensing.