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bioRxiv · 10.1101/657346

High-throughput transmission electron microscopy with automated serial sectioning

Graham, B. J.·Hildebrand, D. G. C.·Kuan, A. T.·Maniates-Selvin, J. T.·Thomas, L. A.·Shanny, B. L.·Lee, W.-C. A.
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Abstract

Transmission electron microscopy (TEM) is an essential tool for studying cells and molecules. We present a tape-based, reel-to-reel pipeline that combines automated serial sectioning with automated high-throughput TEM imaging. This acquisition platform provides nanometer-resolution imaging at fast rates for a fraction of the cost of alternative approaches. We demonstrate the utility of this imaging platform for generating datasets of biological tissues with a focus on examining brain circuits.

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Graham, B. J., Hildebrand, D. G. C., Kuan, A. T., Maniates-Selvin, J. T., Thomas, L. A., Shanny, B. L., Lee, W.-C. A.. 2019-06-02. High-throughput transmission electron microscopy with automated serial sectioning. https://doi.org/10.1101/657346

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