Arabidopsis SWR1-associated protein methyl-CpG-binding domain 9 is required for histone H2A.Z deposition.

Potok, M. E.; Wang, Y.; Xu, L.; Zhong, Z.; Liu, W.; Feng, S.; Naranbaatar, B.; Rayatpisheh, S.; Wang, Z.; Wohlschlegel, J. E.; Ausin, I.; Jacobsen, S. E.

doi:10.1101/657296

bioRxiv · 10.1101/657296

Arabidopsis SWR1-associated protein methyl-CpG-binding domain 9 is required for histone H2A.Z deposition.

Potok, M. E.·Wang, Y.·Xu, L.·Zhong, Z.·Liu, W.·Feng, S.·Naranbaatar, B.·Rayatpisheh, S.·Wang, Z.·Wohlschlegel, J. E.·Ausin, I.·Jacobsen, S. E.

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Abstract

Deposition of the histone variant H2A.Z by the SWI2/SNF2-Related 1 chromatin remodeling complex (SWR1-C) is important for gene regulation in eukaryotes, but the composition of the Arabidopsis SWR1-C has not been thoroughly characterized. Here identify interacting partners of a conserved Arabidopsis SWR1 subunit, ACTIN-RELATED PROTEIN 6 (ARP6). We isolated nine predicted components, and identified additional interactors implicated in histone acetylation and chromatin biology. One of the novel interacting partners, methyl-CpG-binding domain 9 (MBD9), also strongly interacted with the Imitation SWItch (ISWI) chromatin remodeling complex. MBD9 was required for deposition of H2A.Z at a distinct subset of ARP6-dependent loci. MBD9 was preferentially bound to nucleosome-depleted regions at the 5 ends of genes containing high levels of activating histone marks. These data suggest that MBD9 is a SWR1-C interacting protein required for H2A.Z deposition at a subset of actively transcribing genes.

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Potok, M. E., Wang, Y., Xu, L., Zhong, Z., Liu, W., Feng, S., Naranbaatar, B., Rayatpisheh, S., Wang, Z., Wohlschlegel, J. E., Ausin, I., Jacobsen, S. E.. 2019-06-03. Arabidopsis SWR1-associated protein methyl-CpG-binding domain 9 is required for histone H2A.Z deposition.. https://doi.org/10.1101/657296

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