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bioRxiv · 10.1101/653477

High Throughput pMHC-I Tetramer Library Production Using Chaperone Mediated Peptide Exchange

Overall, S. A.·Toor, J. S.·Hao, S.·Yarmarkovich, M.·Nguyen, S.·Japp, A. S.·Moschidi, D.·Betts, M. R.·Maris, J. M.·Smibert, P.·Sgourakis, N. G.
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Abstract

Peptide exchange technologies are essential for the generation of pMHC-multimer libraries, used to probe highly diverse, polyclonal TCR repertoires. Using the molecular chaperone TAPBPR, we present a robust method for the capture of stable, empty MHC-I molecules which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner. Combined with tetramer barcoding using multi-modal cellular indexing technology (ECCITE-seq), our approach allows a combined analysis of TCR repertoires and other T-cell transcription profiles together with their cognate pMHC-I specificities in a single experiment.

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Overall, S. A., Toor, J. S., Hao, S., Yarmarkovich, M., Nguyen, S., Japp, A. S., Moschidi, D., Betts, M. R., Maris, J. M., Smibert, P., Sgourakis, N. G.. 2019-05-30. High Throughput pMHC-I Tetramer Library Production Using Chaperone Mediated Peptide Exchange. https://doi.org/10.1101/653477

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