Simultaneous 3D super-resolution fluorescence microscopy and atomic force microscopy: combined SIM and AFM platform for cell imaging

Gomez-Varela, A. I.; Stamov, D. R.; Miranda, A.; Alves, R.; Barata-Antunes, C.; Dambournet, D.; Drubin, D. G.; Paiva, S.; De Beule, P. A. A.

doi:10.1101/638262

bioRxiv · 10.1101/638262

Simultaneous 3D super-resolution fluorescence microscopy and atomic force microscopy: combined SIM and AFM platform for cell imaging

Gomez-Varela, A. I.·Stamov, D. R.·Miranda, A.·Alves, R.·Barata-Antunes, C.·Dambournet, D.·Drubin, D. G.·Paiva, S.·De Beule, P. A. A.

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Abstract

Correlating data from different microscopy techniques holds the potential to discover new facets of signaling events in cellular biology. Here we report for the first time a hardware set-up capable of achieving simultaneous imaging of spatially correlated super-resolution fluorescence microscopy and atomic force microscopy, a feat only obtained until now by fluorescence microscopy set-ups with spatial resolution restricted to the Abbe resolution limit. We hereby remove the need to perform independent measurement and subsequent data averaging required to eliminate cell-to-cell variation in observed signals. We detail system integration, demonstrate system performance and report imaging of sub-resolution fluorescent beads and genome-engineered human bone osteosarcoma epithelial cells.

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Gomez-Varela, A. I., Stamov, D. R., Miranda, A., Alves, R., Barata-Antunes, C., Dambournet, D., Drubin, D. G., Paiva, S., De Beule, P. A. A.. 2019-05-17. Simultaneous 3D super-resolution fluorescence microscopy and atomic force microscopy: combined SIM and AFM platform for cell imaging. https://doi.org/10.1101/638262

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