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bioRxiv · 10.1101/634592

Characterisation of the c10orf76-PI4KB complex, and its necessity for Golgi PI4P levels and enterovirus replication

McPhail, J. A.·Lyoo, H.·Pemberton, J. G.·Hoffmann, R. M.·van Elst, W.·Strating, J. R. P. M.·Jenkins, M. L.·Stariha, J.·van Kuppeveld, F. J. M.·Balla, T.·Burke, J. E.
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Abstract

The lipid kinase PI4KB, which generates phosphatidylinositol 4-phosphate (PI4P), is a key enzyme in regulating membrane transport and is also hijacked by multiple picornaviruses to mediate viral replication. PI4KB can interact with multiple protein binding partners, which are differentially manipulated by picornaviruses to facilitate replication. The protein c10orf76 is a PI4KB-associated protein that increases PI4P levels at the Golgi, and is essential for the viral replication of specific enteroviruses. We used hydrogen deuterium exchange mass spectrometry to characterize the c10orf76-PI4KB complex and reveal that binding is mediated by the kinase linker of PI4KB, with formation of the heterodimeric complex modulated by PKA-dependent phosphorylation. Complex-disrupting mutations demonstrate that PI4KB is required for membrane recruitment of c10orf76 to the Golgi, and that an intact c10orf76-PI4KB complex is required for the replication of c10orf76-dependent enteroviruses. Intriguingly, c10orf76 was also required for proper Arf1 activation at the Golgi, providing a putative mechanism for the c10orf76-dependent increase in PI4P levels at the Golgi.\n\nHighlightsO_LIc10orf76 forms a direct complex with PI4KB, with the interface formed by a disorder-to-order transition in the kinase linker of PI4KB\nC_LIO_LIThe c10orf76 binding site of PI4KB can be phosphorylated by PKA, with phosphorylation leading to decreased affinity for c10orf76\nC_LIO_LIComplex-disrupting mutants of PI4KB and c10orf76 reveal that PI4KB recruits c10orf76 to the Golgi/TGN\nC_LIO_LIDepletion of c10orf76 leads to decreases in both active Arf1 and Golgi PI4P levels\nC_LIO_LIEnteroviruses that rely on c10orf76 for replication depend on formation of the c10orf76-PI4KB complex\nC_LI

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McPhail, J. A., Lyoo, H., Pemberton, J. G., Hoffmann, R. M., van Elst, W., Strating, J. R. P. M., Jenkins, M. L., Stariha, J., van Kuppeveld, F. J. M., Balla, T., Burke, J. E.. 2019-05-10. Characterisation of the c10orf76-PI4KB complex, and its necessity for Golgi PI4P levels and enterovirus replication. https://doi.org/10.1101/634592

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