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bioRxiv · 10.1101/632315

HYPER-sol: flash-frozen results from archival FFPE tissue for clinical proteomics

Marchione, D. M.·Ilieva, I.·Garcia, B. A.·Pappin, D. J.·Wilson, J. P.·Wojcik, J. B.
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Abstract

Massive formalin-fixed, paraffin-embedded (FFPE) tissue archives exist worldwide, representing a potential gold mine for clinical proteomics research. However, current protocols for FFPE proteomics lack standardization, efficiency, reproducibility, and scalability. Here we present High-Yield Protein Extraction and Recovery by direct SOLubilization (HYPERsol), an optimized workflow using adaptive-focused acoustics (AFA) ultrasonication and S-Trap sample processing that enables proteome coverage and quantification from FFPE samples comparable to that achieved from flash-frozen tissue (average R = 0.936).

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Marchione, D. M., Ilieva, I., Garcia, B. A., Pappin, D. J., Wilson, J. P., Wojcik, J. B.. 2019-05-09. HYPER-sol: flash-frozen results from archival FFPE tissue for clinical proteomics. https://doi.org/10.1101/632315

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