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bioRxiv · 10.1101/630301

Synthetic peptide-induced internalization of biomolecules into various plant and algal cells via micropinocytosis

Chuah, J.-A.·Odahara, M.·Kodama, Y.·Miyamoto, T.·Tsuchiya, K.·Motoda, Y.·Kigawa, T.·Numata, K.
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Abstract

Efficient intracellular delivery of biomolecules is important for many different biological and biotechnological applications in living organisms, and is a prerequisite for certain types of fundamental and applied research. One major challenge is the delivery of unmodified, functional cargoes in a simple, time-efficient, and high-throughput manner. Herein, we present an efficient strategy that uses fusion peptides containing cell penetrating peptide, endosomal escape domain, and a sarcosine linker to introduce biomolecules, namely fluorescent protein and dextran, via macropinocytosis into the cells of various land plants and microalgae. Our peptide-mediated delivery system allows for high-throughput delivery of functional biomolecules within a few minutes to a few hours as well as open new possibilities for biology and biotechnology using difficult-to-transfect cell types.

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Chuah, J.-A., Odahara, M., Kodama, Y., Miyamoto, T., Tsuchiya, K., Motoda, Y., Kigawa, T., Numata, K.. 2019-05-07. Synthetic peptide-induced internalization of biomolecules into various plant and algal cells via micropinocytosis. https://doi.org/10.1101/630301

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