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bioRxiv · 10.1101/621011

TeraVR Empowers Precise Reconstruction of Complete 3-D Neuronal Morphology in the Whole Brain

Wang, Y.·Li, Q.·Liu, L.·Zhou, Z.·Kong, L.·Zhong, N.·Chai, R.·Luo, X.·Guo, Y.·Hawrylycz, M.·Luo, Q.·Gu, Z.·Xie, W.·Zeng, H.·Peng, H.
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Abstract

Neuron morphology is recognized as a key determinant of cell type, yet the quantitative profiling of a mammalian neurons complete three-dimensional (3-D) morphology remains arduous when the neuron has complex arborization and long projection. Whole-brain reconstruction of neuron morphology is even more challenging as it involves processing tens of teravoxels of imaging data. Validating such reconstructions is extremely laborious. We developed TeraVR, an open-source virtual reality annotation system, to address these challenges. TeraVR integrates immersive and collaborative 3-D visualization, interaction, and hierarchical streaming of teravoxel-scale images. Using TeraVR, we produced precise 3-D full morphology of long-projecting neurons in whole mouse brains and developed a collaborative workflow for highly accurate neuronal reconstruction.

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Wang, Y., Li, Q., Liu, L., Zhou, Z., Kong, L., Zhong, N., Chai, R., Luo, X., Guo, Y., Hawrylycz, M., Luo, Q., Gu, Z., Xie, W., Zeng, H., Peng, H.. 2019-04-28. TeraVR Empowers Precise Reconstruction of Complete 3-D Neuronal Morphology in the Whole Brain. https://doi.org/10.1101/621011

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