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bioRxiv · 10.1101/620906

Effect of mannose modified chitosan on uptake of nanoparticles by macrophages

Smith, J.·Lowsberg, D.
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Abstract

This report provided a new method to prepare and evaluate mannose-modified chitosan-coated lactic acid-glycolic acid copolymer (PLGA) nanoparticles, and to investigate their effects on macrophage toxicity and macrophage uptake. The PLGA nanoparticles loaded with ovalbumin (OVA) were prepared by double emulsion method. The size and zeta potential of the nanoparticles were determined by laser granulometry after mannose-modified chitosan coating. The nanoparticles were observed by transmission electron microscopy. The appearance of the form, the BCA method to determine the OVA content, calculate the drug loading and release. The OVA nanoparticles labeled with fluorescein isothiocyanate (FITC) were co-incubated with macrophages (RAW 264. 7), cell viability was determined by MTT assay, and uptake was examined by fluorescence microscopy. Results The size and {zeta} potential of OVA-PLGA nanoparticles increased with the increase of chitosan coating concentration (P < 0.05), and OVA drug loading range was 7. 2% to 8. 4%. Chitosan and mannose modified chitosan coating FITC-OVAPLGA nanoparticles and RAW 264. 7 After incubation, there was little effect on cell viability (P > 0.05), but it significantly promoted macrophage uptake by FITC-OVA-PLGA nanoparticles (P < 0.05).

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Smith, J., Lowsberg, D.. 2019-04-27. Effect of mannose modified chitosan on uptake of nanoparticles by macrophages. https://doi.org/10.1101/620906

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