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bioRxiv · 10.1101/617837

Tracking the cells of tumor origin in breast organoids by light sheet microscopy

Alladin, A.·Chaible, L.·Reither, S.·Loeschinger, M.·Wachsmuth, M.·Heriche, J.-K.·Tischer, C.·Jechlinger, M.
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Abstract

How tumors arise from individual transformed cells within an intact epithelium is a central, yet unanswered question. Here, we developed a new methodology that combines breast tissue organoids, where oncogenes can be switched on in single cells, with light-sheet imaging that allows us to track cell fates using a big-image-data analysis workflow. The power of this integrated approach is illustrated by our finding that small local groups of transformed cells form tumors while isolated transformed cells do not.

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Alladin, A., Chaible, L., Reither, S., Loeschinger, M., Wachsmuth, M., Heriche, J.-K., Tischer, C., Jechlinger, M.. 2019-04-24. Tracking the cells of tumor origin in breast organoids by light sheet microscopy. https://doi.org/10.1101/617837

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