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bioRxiv · 10.1101/616748

PSC-RED, an Albumin-Free Robust Erythroid Differentiation Method to Produce Enucleated Red Blood Cells from Human Pluripotent Stem Cells

Olivier, E. E.·Zhang, S.·Yan, Z.·Suzuka, S.·Roberts, K. E.·Wang, K. E.·Bouhassira, E. E.
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Abstract

Cultured red blood cells (cRBCs) have many potential applications in transfusion medicine and drug delivery. We report that we have developed chemically defined, albumin-free Robust Erythroid Differentiation (RED) methods to produce enucleated cRBCs from human induced pluripotent stem cells (iPSCs). Human iPSC-derived cRBCs produced with either the short or long variation of the RED protocol respectively express embryonic/fetal or a mixture of fetal and adult hemoglobins. The long version of the protocol produces up to 50% of enucleated cells at an unprecedented yield. RED is scalable and relies on inexpensive components and therefore dramatically increases the feasibility and economic viability of all translational applications of cRBCs.\n\nHighlightsO_LIPSC-RED: A chemically-defined, albumin-free Robust Erythroid Differentiation (RED) methods to produce cRBCs from human induced pluripotent stem cells.\nC_LIO_LIPSC-RED produces up to 50% enucleated cells at an unprecedented yield.\nC_LIO_LIPSC-RED is scalable and relies on inexpensive components and therefore increases the feasibility and economic viability of translational applications of cRBCs.\nC_LI

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Olivier, E. E., Zhang, S., Yan, Z., Suzuka, S., Roberts, K. E., Wang, K. E., Bouhassira, E. E.. 2019-04-23. PSC-RED, an Albumin-Free Robust Erythroid Differentiation Method to Produce Enucleated Red Blood Cells from Human Pluripotent Stem Cells. https://doi.org/10.1101/616748

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