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bioRxiv · 10.1101/611798

Induction of Chk2 signaling in the germarium is sufficient to cause oogenesis arrest in Drosophila

Ephrussi, A.·Durdevic, Z.
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Abstract

The conserved RNA helicase Vasa is required for germ cell development in many organisms. It is established that in Drosophila loss of piRNA pathway components, including Vasa, causes Chk2-dependent oogenesis arrest, however the stage at which Chk2-signaling is triggered was unknown. We found that absence of Vasa during the germarial stages arrests oogenesis due to Chk2 activation. Importantly, once induced in the germarium, Chk2-mediated arrest of oogenesis cannot be overcome by restoration of Vasa to the arrested egg-chambers. We conclude that Vasa activity specifically in the germarium is essential for germ cell lineage development.

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Ephrussi, A., Durdevic, Z.. 2019-04-17. Induction of Chk2 signaling in the germarium is sufficient to cause oogenesis arrest in Drosophila. https://doi.org/10.1101/611798

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