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bioRxiv · 10.1101/609255

Proteoliposomes as energy transferring nanomaterials: enhancing the spectral range of light-harvesting proteins using lipid-linked chromophores

Hancock, A. M.·Meredith, S. A.·Connell, S. D. A.·Jeuken, L. J. C.·Adams, P. G.
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Abstract

Biology provides a suite of optically-active nanomaterials in the form of \"light harvesting\" protein-chlorophyll complexes, however, these have drawbacks including their limited spectral range. We report the generation of model lipid membranes (proteoliposomes) incorporating the photosynthetic protein Light-Harvesting Complex II (LHCII) and lipid-tethered Texas Red (TR) chromophores that act as a \"bio-hybrid\" energy transferring nanomaterial. The effective spectral range of the protein is enhanced due to highly efficient energy transfer from the TR chromophores (up to 94%), producing a marked increase in LHCII fluorescence (up to 3x). Our self-assembly procedure offers excellent modularity allowing the incorporation of a range of concentrations of energy donors (TR) and acceptors (LHCII), allowing the energy transfer efficiency (ETE) and LHCII fluorescence to be tuned as desired. Fluorescence Lifetime Imaging Microscopy (FLIM) provides single-proteoliposome-level quantification of ETE, revealing distributions within the population and proving that functionality is maintained on a surface. Our membrane-based system acts as a controllable light harvesting nanomaterial with potential applications as thin films in photo-active devices.\n\n\n\nO_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=92 SRC=\"FIGDIR/small/609255v1_ufig1.gif\" ALT=\"Figure 1\">\nView larger version (32K):\norg.highwire.dtl.DTLVardef@e663f3org.highwire.dtl.DTLVardef@1abfa78org.highwire.dtl.DTLVardef@1c90d5aorg.highwire.dtl.DTLVardef@8b99db_HPS_FORMAT_FIGEXP M_FIG Table of Contents Figure C_FIG

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Hancock, A. M., Meredith, S. A., Connell, S. D. A., Jeuken, L. J. C., Adams, P. G.. 2019-04-25. Proteoliposomes as energy transferring nanomaterials: enhancing the spectral range of light-harvesting proteins using lipid-linked chromophores. https://doi.org/10.1101/609255

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