Light-sheet microscopy with isotropic, sub-micron resolution and solvent-independent large-scale imaging

Chakraborty, T.; Driscoll, M.; Murphy, M. M.; Roudot, P.; Chang, B.-J.; Vora, S.; Wong, W. M.; Nielson, C.; Zhang, H.; Zhemkov, V.; Hiremath, C.; De La Cruz, E. D.; Bezprozvanny, I.; Zhao, H.; Tomer, R.; Heintzmann, R.; Meeks, J.; Marciano, D.; Morrison, S.; Danuser, G.; Dean, K.; Fiolka, R.

doi:10.1101/605493

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bioRxiv · 10.1101/605493

Light-sheet microscopy with isotropic, sub-micron resolution and solvent-independent large-scale imaging

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Abstract

We present cleared tissue Axially Swept Light-Sheet Microscopy (ctASLM), which achieves sub-micron isotropic resolution, high optical sectioning capability, and large field of view imaging (870x870 m2) over a broad range of immersion media. ctASLM can image live, expanded, and both aqueous and organic chemically cleared tissue preparations and provides 2- to 5-fold better axial resolution than confocal or other reported cleared tissue light-sheet microscopes. We image millimeter-sized tissues with sub-micron 3D resolution, which enabled us to perform automated detection of cells and subcellular features such as dendritic spines.

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Chakraborty, T., Driscoll, M., Murphy, M. M., Roudot, P., Chang, B.-J., Vora, S., Wong, W. M., Nielson, C., Zhang, H., Zhemkov, V., Hiremath, C., De La Cruz, E. D., Bezprozvanny, I., Zhao, H., Tomer, R., Heintzmann, R., Meeks, J., Marciano, D., Morrison, S., Danuser, G., Dean, K., Fiolka, R.. 2019-04-11. Light-sheet microscopy with isotropic, sub-micron resolution and solvent-independent large-scale imaging. https://doi.org/10.1101/605493

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