Induced expression of Xerophyta viscosa XvSap1 gene greatly impacts tolerance to drought stress in transgenic sweetpotato

Abstract

Key message Drought stress in sweetpotato could be overcome by introducing XvSap1 gene from Xerophyta viscosa.\n\nDrought stress often leads to reduced yields and is perilous delimiter for expanded cultivation and increased productivity of sweetpotato. Cell wall stabilization proteins have been identified to play a pivotal role in mechanical stabilization during desiccation stress mitigation. They are involved in myriad cellular processes that modify the cell wall properties to tolerate the mechanical stress during dehydration in plants. This provides a possible approach to engineer crops for enhanced stable yields under adverse climatic conditions. In this study, we introduced the XvSap1 gene isolated from Xerophyta viscosa, a resurrection plant into sweetpotato by Agrobacterium-mediated transformation. Detection of the transgene by PCR coupled with Southern blot revealed the integration of XvSap1 in the three independent events. Sweetpotato plants expressing the XvSap1 gene exhibited superior growth performance such as shoot length, number of leaves and yield than the wild type plants under drought stress. Quantitative real time-PCR results confirmed higher expression of the XvSap1 gene in XSP1 transgenic plants imposed with drought stress. In addition, the transgenic plants had increased levels of chlorophyll, free proline and relative water content but malonaldehyde content was decreased under drought stress compared to wild type plants. Conjointly, our findings show that XvSap1 can enhance drought resilience without causing deleterious phenotypic and yield changes, thus providing a promising candidate target for improving the drought tolerance of sweetpotato cultivars through genetic engineering. The transgenic drought tolerant sweetpotato line provides a valuable resource as drought tolerant crop on arid lands of the world.