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bioRxiv · 10.1101/600262

Serum proteomic approach for differentiation of frail and non-frail elderly

Dey, S.·Agnihotri, V.·Gupta, A.·Pradhan, R.·Venugopalan, G. V.·Bajpai, S.·Dey, A. B.
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Abstract

Frail elderly is very common in Indian society and extremely difficult to manage in the clinical practice. Blood proteomic study may able to improve the specific diagnostic profiles and therapeutic templates for improving quality of life in elderly. The purpose of present study is to differentiate between frail and non-frail elderly on the basis of serum markers. The proteomic profile of 10 frail and 10 non-frail elderly diagnosed according to deficit accumulation model of Rockwood was identified by 2D-electrophoresis and analyzed using ImageMaster 2DPlatinum7.0 software. The proteins were identified by MALDI-TOF/TOF and performed gene ontology study by PANTHER 7.0 software. Overall 105 spots were identified in the study groups. In frail 22spots and in non-frail 12spots were found to be differentially expressed. Mass spectroscopy analysis of 13 spots showed up-regulated Haptoglobin, Serum amyloidA1, TRAK1, sp110, NLRC3, MMP12, Mortalin, NDK3 and downregulated PSRC1, NKG2A proteins in frail elderly. The differential expression of proteins in frailty are mostly associated with pro-inflammation and is well-known that frailty syndrome increases all inflammatory parameters. It can be summarized from the present proteomic study that these proteins can be used as potential biomarkers for early detection of frailty in the elderly.

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Dey, S., Agnihotri, V., Gupta, A., Pradhan, R., Venugopalan, G. V., Bajpai, S., Dey, A. B.. 2019-04-05. Serum proteomic approach for differentiation of frail and non-frail elderly. https://doi.org/10.1101/600262

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