Nanog regulates Pou3f1 expression and represses anterior fate at the exit from pluripotency.

Barral, A.; Rollan, I.; Sanchez-Iranzo, H.; Jawaid, W.; Badia-Careaga, C.; Menchero, S.; Gomez-Rodriguez, M.; Torroja, C.; Sanchez-Cabo, F.; Gottgens, B.; Manzanares, M.; Sainz de Aja, J.

doi:10.1101/598656

bioRxiv · 10.1101/598656

Nanog regulates Pou3f1 expression and represses anterior fate at the exit from pluripotency.

Barral, A.·Rollan, I.·Sanchez-Iranzo, H.·Jawaid, W.·Badia-Careaga, C.·Menchero, S.·Gomez-Rodriguez, M.·Torroja, C.·Sanchez-Cabo, F.·Gottgens, B.·Manzanares, M.·Sainz de Aja, J.

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Abstract

Pluripotency is regulated by a network of transcription factors that maintains early embryonic cells in an undifferentiated state while allowing them to proliferate. NANOG is a critical factor for maintaining pluripotency and its role in primordial germ cell differentiation has been well described. However, Nanog is expressed during gastrulation across all the posterior epiblast, and only later in development its expression is restricted to primordial germ cells. In this work, we unveiled a previously unknown mechanism by which Nanog specifically represses the anterior epiblast lineage. Analysis of transcriptional data from both embryonic stem cells and gastrulating mouse embryos revealed Pou3f1 expression to be negatively correlated with that of Nanog during the early stages of differentiation. We have functionally demonstrated Pou3f1 to be a direct target of NANOG by using a dual transgene system for the controlled expression of Nanog. Use of Nanog null ES cells further demonstrated a role for Nanog in repressing anterior neural genes. Deletion of a NANOG binding site (BS) located nine kilobases downstream of the transcription start site of Pou3f1 revealed this BS to have a specific role in the regionalization of the expression of this gene in the embryo. Our results indicate an active role of Nanog inhibiting the neural fate by repressing Pou3f1 at the onset of gastrulation.

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Barral, A., Rollan, I., Sanchez-Iranzo, H., Jawaid, W., Badia-Careaga, C., Menchero, S., Gomez-Rodriguez, M., Torroja, C., Sanchez-Cabo, F., Gottgens, B., Manzanares, M., Sainz de Aja, J.. 2019-04-04. Nanog regulates Pou3f1 expression and represses anterior fate at the exit from pluripotency.. https://doi.org/10.1101/598656

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