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bioRxiv · 10.1101/597971

A simple and efficient CRISPR technique for protein tagging

Zeng, F.·Beck, V.·Schuierer, S.·Garnier, I.·Manneville, C.·Agarinis, C.·Morelli, L.·Quinn, L.·Knehr, J.·Roma, G.·Bassilana, F.·Nash, M. S.
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Abstract

Genetic knock-in using homology directed repair is an inefficient process, requiring selection of few modified cells and hindering its application to primary cells. Here we describe Homology independent gene Tagging (HiTag), a method to tag a protein of interest by CRISPR in up to 66% transfected cells with one single electroporation. The technique has proven effective in various cell types, can be used to knock in a fluorescent protein for live cell imaging, to modify the cellular location of a target protein and to monitor levels of a protein of interest by a luciferase assay in primary cells.

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Zeng, F., Beck, V., Schuierer, S., Garnier, I., Manneville, C., Agarinis, C., Morelli, L., Quinn, L., Knehr, J., Roma, G., Bassilana, F., Nash, M. S.. 2019-04-05. A simple and efficient CRISPR technique for protein tagging. https://doi.org/10.1101/597971

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