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bioRxiv · 10.1101/587386

A tissue-like platform for studying engineered quiescent human T-cells’ interactions with dendritic cells

Abu-Shah, E.·Demetriou, P.·Mayya, V.·Balint, S.·Kutuzov, M. A.·Dushek, O.·Dustin, M. L.
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Abstract

Research in the field of human immunology is restricted by the lack of a system that reconstitutes the in-situ activation dynamics of quiescent human antigen-specific T-cells interacting with dendritic cells. Here we report a tissue-like system that recapitulates the dynamics of engineered primary human immune cell. Our approach facilitates real-time single cell manipulations, tracking of interactions and functional responses complemented by population-based measurements of cytokines, activation status and proliferation. As a proof of concept, we recapitulate immunological phenomenon such as CD4 help to CD8 T-cells through enhanced maturation of DCs and effect of PD-1 checkpoint blockades. In addition, we characterise unique dynamics of T-cell/DC interactions as a function of antigen affinity.

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Abu-Shah, E., Demetriou, P., Mayya, V., Balint, S., Kutuzov, M. A., Dushek, O., Dustin, M. L.. 2019-03-24. A tissue-like platform for studying engineered quiescent human T-cells’ interactions with dendritic cells. https://doi.org/10.1101/587386

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