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bioRxiv · 10.1101/584052

Inhibition of Osteoblast Proliferation and Migration by Exogenous and Endogenous Formaldehyde

Teng, X.·Huang, W.·Yu, H.·Wang, P.·Li, W.·Chen, Z.·Fan, D.
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Abstract

Exogenous and endogenous formaldehyde (FA) plays an important role in cell growth and migration; however, its potential role in osteoblasts remains largely unclear. Cell counting kit-8 (CCK-8) and wound healing assays revealed that FA exposure at naturally occurring concentrations inhibited the proliferation and migration of mouse preosteoblast MC3T3-E1 cells. Moreover, RNA sequencing (RNA-seq) analysis revealed that FoxO1 signaling pathway components displayed distinct expression patterns upon FA exposure, reflected through significant enrichment of cell migration. In particular, FoxO1 Sirt1 and FA-induced related protein expression which were closely with cell proliferation and migration were confirmed by western blotting. The present results indicate that the FoxO1 pathway is involved in FA-induced inhibition of cell growth and migration.

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Teng, X., Huang, W., Yu, H., Wang, P., Li, W., Chen, Z., Fan, D.. 2019-03-20. Inhibition of Osteoblast Proliferation and Migration by Exogenous and Endogenous Formaldehyde. https://doi.org/10.1101/584052

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