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bioRxiv · 10.1101/583633

Krüppel-like factor 2- induced microRNAs: implications for treatment of pulmonary hypertension

Abstract

Flow-activated transcription factor Kruppel-like factor 2 (KLF2) signaling is compromised in pulmonary arterial hypertension (PAH). We aimed to identify KLF2-induced endothelium-protective exosomal microRNAs of potential therapeutic significance.\n\nEight exosomal microRNAs elevated by KLF2 but reduced in PAH were transfected into human pulmonary artery endothelial cells. Of these, only miR-181a-5p and miR-324-5p had anti-apoptotic, anti-inflammatory and anti-proliferative effect on endothelial cells and reduced proliferation of vascular smooth muscle cells in vitro. RNA sequencing of miRNA-transfected HPAECs revealed reduced expression of multiple genes implicated in vascular remodelling, including ETS-1, NOTCH4, ACTA2, TNF-, IL-1, MMP10, MAPK and NFATC2. KLF2, miR-181a-5p and miR-324-5p were reduced, while their target genes were elevated in blood-derived endothelial colony forming cells and lung tissues from idiopathic and heritable PAH patients with disabling KLF2 mutation and Sugen/hypoxia mice. Supplementation of miR-181a-5p and miR-324-5p or silencing of their target genes attenuated proliferative and angiogenic responses in endothelial cells from idiopathic PAH and prevented development of pulmonary hypertension in Sugen/hypoxia mice.\n\nThis study highlights potential therapeutic role of KLF2-induced exosomal microRNAs in PAH.

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BibTeXRIS

Sindi, H., Russomanno, G., Kyeong Beom, J., Abdul-Salam, V. B., Chaudhry, B. Q., Ainscough, A., Szulcek, R., Bogaard, H. J., Morgan, C., Pullamsetti, S. S., Alzaydi, M., Rhodes, C., Grünig, E., Eichstaedt, C., Wilkins, M. R., Wojciak-Stothard, B.. 2019-03-21. Krüppel-like factor 2- induced microRNAs: implications for treatment of pulmonary hypertension. https://doi.org/10.1101/583633

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