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bioRxiv · 10.1101/575449

Ligand-independent role of ErbB3 in endocytotic recycling

Saez-Ibanez, A. R.·Scheffler, J. M.·Terabayashi, T.·Idevall, O.·Ferby, I.
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Abstract

Receptor tyrosine kinases (RTKs) are important cargo in endocytic trafficking, yet their role in endosomal sorting and maturation of multivesicular bodies (MVBs) remains unclear. Here we show that the ErbB3 receptor sorts internalised Integrin {beta}1, and the transferrin receptor (TfR), for endocytic recycling, in a manner that does not require ligand-induced ErbB3 signaling in breast epithelial cells. Loss of ErbB3 abrogates recycling of Integrin {beta}1, likely from a Rab4-positive compartment, and redirects it toward lysosomal degradation and secretion as extracellular vesicle (EV) cargo. ErbB3 depletion impairs the collective migration of breast epithelial cell sheets, coinciding with reduced cell surface levels of Integrin {beta}1 and increased release of Integrin {beta}1-containing EVs. In contrast, EVs secreted from ErbB3-depleted cells enhance the motility of wild-type cells. Mechanistically, ErbB3 promotes assembly of the Arf6-GGA3-Rabaptin5 endosomal sorting complex to facilitate early recycling and suppress EV release. These findings provoke the notion that pseudo-RTKs play an active role in vesicular trafficking.

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Saez-Ibanez, A. R., Scheffler, J. M., Terabayashi, T., Idevall, O., Ferby, I.. 2019-03-13. Ligand-independent role of ErbB3 in endocytotic recycling. https://doi.org/10.1101/575449

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