Genetic diversity of clinical Bordetella pertussis ST2 strains in comparison with vaccine reference strains of India

Sharma, N. C.; Anandan, S.; Devanga Ragupathi, N. K.; Muthuirulandi Sethuvel, D. P.; Vasudevan, K.; Kumar, D.; Gupta, S. K.; Sangal, L.; Murugesan, V.; Veeraraghavan, B.

doi:10.1101/573543

bioRxiv · 10.1101/573543

Genetic diversity of clinical Bordetella pertussis ST2 strains in comparison with vaccine reference strains of India

Sharma, N. C.·Anandan, S.·Devanga Ragupathi, N. K.·Muthuirulandi Sethuvel, D. P.·Vasudevan, K.·Kumar, D.·Gupta, S. K.·Sangal, L.·Murugesan, V.·Veeraraghavan, B.

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Abstract

Pertussis is a highly contagious disease of the respiratory tract caused by Bordetella pertussis, a bacteria that lives in the mouth, nose, and throat. Current study reports the highly accurate complete genomes of two clinical B. pertussis strains from India for the first time. The analysis revealed insertional elements flanked by IS481, which has been previously regarded as the important component for bacterial evolution. The two B. pertussis clinical strains exhibited diversity through genome degradation when compared to whole-cell pertussis vaccine reference strains of India. These isolates harboured multiple genetic virulence traits and toxin subunits, which belonged to sequence type ST2. The genome information of Indian clinical B. pertussis strains will serve as a baseline data to decipher more information on the genome evolution, virulence factors and their role in pathogenesis for effective vaccine strategies.

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Sharma, N. C., Anandan, S., Devanga Ragupathi, N. K., Muthuirulandi Sethuvel, D. P., Vasudevan, K., Kumar, D., Gupta, S. K., Sangal, L., Murugesan, V., Veeraraghavan, B.. 2019-03-13. Genetic diversity of clinical Bordetella pertussis ST2 strains in comparison with vaccine reference strains of India. https://doi.org/10.1101/573543

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